In this context, one first approach to assess the contribution of upregulated Myosin 1 g is by evaluating the molecular interactions with the barriers in CNS and testis and migration mechanism either using B-ALL cells from patients or B cell lines overexpressing Myosin 1 g in human-derived in vitro models that recapitulate the crossing through barriers of CNS and testis. The gene discussed is MYH1; the disease is acute lymphoblastic leukemia.