To address whether MORC2 O-GlcNAcylation is involved in TGF-β1-mediated breast cancer cell migration and invasion, we reconstituted empty vector pMSCV or Flag-MORC2 (WT or T556A) into MORC2-KO LM2–4175 and BT549 cells by lentiviral infection and then treated with or without TGF-β1 (Supplementary Fig. S5C). This evidence concerns the gene TGFB1 and breast carcinoma.