To confirm the array data, we detected TRIB2 mRNA expression by RT-PCR, and Western blot showed strong upregulation of TRIB2 protein levels in enzalutamide-resistant (LNCaP-ENR, PCa-2B-ENR, and LAPC4-ENR) prostate cancer cells (Fig. 1, E and F). This evidence concerns the gene TRIB2 and Familial prostate cancer.