Because we must further evaluate our mechanism using propionate-producing bacteria, we selected the BT supernatant and confirmed the HECTD2-EHMT2-TNFAIP1 axis by BT sup treatment, implying that propionate-producing bacteria in the gut microbiota, including BT, may suppress the proliferation of colon cancer and induce cell apoptosis. Here, HECTD2 is linked to malignant colon neoplasm.