Although we did not observe differences in the E7-specific CD8+T cell response in E7 RNA-LPX and E7 RNA-LPX/LRT-treated mice five days after LRT (Fig. 2d, e), the reduction of tumor cell count, tumor hypoxia and increased CD8+ TIL proliferation led us to investigate the antigen-specific CD8+T cell response when tumor growth curves diverged more strongly, namely day 29 after tumor injection (Fig. 4a) and hypotheized that a more oxygenated environment can have subsequent effects on immune cell types, such as T cells. This evidence concerns the gene CD8A and neoplasm.