CD8A and neoplasm: To identify if LRT sensitizes TC-1 tumors to E7-specific CD8+T cell-induced death, we co-cultured differentially irradiated TC-1 tumor cells (0 to 12 Gy) with CD8+T cells isolated from spleens of E7 RNA-LPX-vaccinated mice in a controlled manner in vitro and measured the expression of MHC class I, PD-L1 and CC3 on tumor cells and the secretion of effector cytokines by E7-specific CD8+T cells (Supplementary Fig. 3).