Therefore, we characterized functional parameters of E7-specific CD8+T cell responses such as total tumor infiltration, the secretion of cytokines involved in T cell effector function (IFNγ, TNFα) and proliferation (IL-2), as well as the expression of negative immune checkpoints (TIM-3, PD-1 and NKG2AB [a Qa-1b ligand [37]) (Fig. 4). This evidence concerns the gene CD8A and neoplasm.