To assess additional effects of Ndufa4l2-depletion, we next stained kidney cortices from three TANdu mice for the ccRCC biomarkers Car9 and Eno1 (red), and concomitantly counterstained with GFP (green) to identify tubules positive for Ndufa4l2-shRNA expression (Figure 4). This evidence concerns the gene ENO1 and nonpapillary renal cell carcinoma.