Based on the dual antitumor properties of the NK4 gene and the high expression of Wnt1/β-Catenin signaling pathway in the head and neck, this study aims to explore the following issues: (1) NK4 expression in transfected LSCC AMC-HN-8 cells; (2) the effects of the NK4 gene on the proliferation, migration, apoptosis and cell cycle of LSCC AMC-HN-8 cells;(3) The effect of NK4 gene on the tumorigenesis ability of nude mice of laryngeal cancer cells, and to explore whether its DKK1/Wnt1/β-Catenin signaling pathway is involved in the effect of NK4 gene on laryngeal cancer. Here, DKK1 is linked to laryngeal carcinoma.