In vitro rh-IL2 activation generated a significant increase in the CD3+IFNγ+IL17+ cell subpopulation for HC and RRMS subjects which, simultaneous with the evolution of CD3+CD161+ and CD3+CXCR3+ cells, was related to CD3+TBet+RORγt+ signaling at day 7 and day 14 of culture. This evidence concerns the gene KLRB1 and relapsing-remitting multiple sclerosis.