Briefly, this human A549 lung adenocarcinoma derived line expresses a membrane-bound anti-CD3 single-chain antibody (A549-SC3 cells) for T cell engagement and both MHC-II and PD-L1 which can provide inhibitory interactions through LAG3 and PD1 respectively on the T cells (Fig. 2a). Here, CD274 is linked to lung adenocarcinoma.