CD34 and acute myeloid leukemia: To test the limits of UPSIDE’s ability, we chose four cell types that, despite having distinct size, shape and textural features, were similar in their gross morphologies (Figs 2A and S3A): a mouse T cell leukemia line (Scid.ADH2), a mouse macrophage cell line (Raw246.7), a human acute myeloid leukemia cell line (Kasumi-1), and primary patient-derived human acute myeloid leukemia stem cells (CD34+CD38- AML LSC).