In addition, extensive methylation profiling of the X chromosome by bisulfite sequencing revealed a total of 20, 15, and nine distinct gene promoters with a significant difference in the methylation profile in CD4(+), CD8(+), and CD14(+) cells in PBC patients, respectively, further implicating epigenetic alterations in disease pathogenesis [51,66]. Here, CD4 is linked to primary biliary cholangitis.