In order to validate whether hsa-miR-582-5p/HMGB1 axis was involved in lncRNA DCST1-AS1 mediated CRC cell proliferation and motility, we further compared the HMGB1 expression level, cell proliferation, invasion, and migration of different treatment groups (In HT-29 cells: sh-NC, sh-lncRNA DCST1-AS1#1, sh-lncRNA DCST1-AS1#1+ hsa-miR-582-5p inhibitor, sh-lncRNA DCST1-AS1#1+ OE-HMGB1; In HCT116 cells: empty vector, DCST1-AS1 overexpression plasmid, DCST1-AS1+ hsa-miR-582-5p mimic, DCST1-AS1+ HMGB1 siRNA). Here, DCST1 is linked to colorectal carcinoma.