To functionally confirm the role of lncRNA DCST1-AS1 in the progression of CRC, lentiviruses carrying shRNA targeting lncRNA DCST1-AS1 (sh-lncRNA DCST1-AS1#1, sh-lncRNA DCST1-AS1#2) were used to stably knock down lncRNA DCST1-AS1, and pcDNA3.1-DCST1-AS1 expression plasmids were used to overexpress DCST1-AS1. Here, DCST1 is linked to colorectal carcinoma.