To confirm that the decrease of HAPLN1 mRNA translates to reduced protein levels in CRC patients, we assessed the HAPLN1 protein in tumor and normal tissues adjacent to tumor tissues from 59 CRC patients using immunohistochemistry (Figure 2A), and the HAPLN1 protein was mainly produced by CRC epithelial cells. The gene discussed is HAPLN1; the disease is colorectal carcinoma.