To ascertain the biological function of RASAL2 in PCa, the LNCaP (androgen-dependent) and PC3 (androgen-independent) cells we transfected pCMV6–RASAL2 (encoding the full length of Rasal2 complete ORF with an expression Myc-DDK tag) or pCMV empty vector as a negative control (Figure 2A and 2B). This evidence concerns the gene RASAL2 and posterior cortical atrophy.