We found that bacteria enriched in HFD group such as Deferribacteraceae, Ruminococcaceae, and Desulfovibrionaceae were positively correlated with metabolic parameters associated with NAFLD (increased body weight; serum levels of ALT, AST, LBP and faecal level of H2S; and decreased level of SOD), while bacteria enriched in the NCD and JZG group such as S24_7, Lachnospiraceae, and Bifidobacteriaceae were negatively correlated with NAFLD-associated metabolic parameters. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.