Then, we found that 43 TFs existed with genetic alterations in BC with the cBioPortal resource based on TCGA data, among which ARNT, ATF3, ESR1, TFAP2C, TP53, and ZNF217 were observed to appear with higher rates of genetic alteration (>5%), and the main alteration type of them was amplification except for TP53 (Supplementary Figure S4). The gene discussed is ESR1; the disease is breast cancer.