In the validation cohort, CK20 detected CRC with 36.7% sensitivity and 88.3% specificity; hyper-methylated CLIP4 with 80% sensitivity and 85% specificity; and CK20/hyper-methylated CLIP4 with 95% sensitivity and 81.7% specificity (Table 4). This evidence concerns the gene KRT20 and colorectal carcinoma.