This growth promoting ability of CLDN2 in colorectal cancer was further confirmed by subsequent CCK8 proliferation assay, where HT29sgCLDN2 cells were less proliferative than control cells (HT29V2) and HCT116CLDN2‐GFP cells were more proliferative than control cells (HCT116GFP) (Figure 2E). The gene discussed is CLDN2; the disease is colorectal cancer.