Moreover, AAV-anti-Mir20b significantly reduced the expression of genes related to hepatic inflammation, including Tnf and Il6 (Figure 8N), and fibrosis, including the NASH-relevant genes, such as Acta2, Col1a1, Col3a1 and Timp1 (Figure 8O), in MCD-fed mice. The gene discussed is COL3A1; the disease is metabolic dysfunction-associated steatohepatitis.