Based on the previous study, this study aims to design novel NBs, study their response to ultrasound irradiation and transfection efficiency, and confirm the hypothesis that PDLIM5 siRNA combined with ultrasound irradiation can downregulate PDLIM5 expression in NSCLC and inhibit tumor growth in vitro by the mechanism of EGFR-TKIS resistance. The gene discussed is PDLIM5; the disease is neoplasm.