We reanalyzed mouse ALS astrocytes carrying the SOD1G93A transgene mutation (three mutant and three wild-type mice) (Guttenplan et al. 2020), astrocyte-specific knockout of Tardbp (using GFAP-Cre recombinase promoter Tardbp knockout; four knockout and four control mice) (Peng et al. 2020), and motor cortex astrocyte-specific Tmem259 knockout (five knockout and five control mice) (Jiang et al. 2019) (Fig. 4A). Here, TMEM259 is linked to amyotrophic lateral sclerosis.