It’s notable that FMNL3-V5 overexpression did not increase colony uptake compared to WT U937 MFs which express much lower amounts of FMNL3 (Fig 8E) indicating that endogenous levels of FMNL3 are sufficient for maximal uptake and the 50% increase in the FMNL3 transcripts induced by gonococcal infection (Fig 8B) might not be biologically meaningful. This evidence concerns the gene FMNL3 and gonorrhea.