The second objective was to investigate whether OCD chondrocytes respond to biological and mechanical stimuli previously reported to be effective on chondrocytes from other animal species and humans.21,25,30,33 We performed a full-factorial study exploring the effects of the application of biological (transforming growth factor beta -1 (TGF-β1), chondroitinase ABC (C-ABC), and Lysyl Oxidase L2 (LOXL2), mechanical (static compression), and a combination of biological and mechanical stimuli on the functional properties of OCD-chon-sourced scaffold-free TE neocartilage constructs. This evidence concerns the gene LOXL2 and osteochondritis dissecans.