Moreover, univariate and multivariate Cox analysis from 228 HCC patients in TCGA data proved that MCM4 was an independent hazardous factor for HCC patients' survival (univariate Cox: HR = 1.614, 95%CI = 1.266-2.056, p < 0.001; multivariate Cox: HR = 1.529, 95%CI = 1.164-2.009, p = 0.002). This evidence concerns the gene MCM4 and hepatocellular carcinoma.