According to the MCM4 and AFP expression levels from 360 HCC tumor tissues and 49 adjacent tissue data in TCGA, as an HCC diagnosis biomarker, MCM4 got a higher AUC of ROC curve than AFP, which showed better sensitivity and specificity (AUC(MCM4) = 0.9461, AUC(AFP) = 0.7056, Figure 3(a)). The gene discussed is AFP; the disease is hepatocellular carcinoma.