Since the overexpression of the autophagic genes ATG5 and ATG7 in sarcoma 180 cells was induced, we performed monodansylcadaverine staining to verify whether the formation of autophagic vacuoles was induced by the treatment with complex 1 at 1 μM, 4 μM and 20 μM. This evidence concerns the gene ATG5 and sarcoma.