ATG7 and sarcoma: In this context, we treated melanoma B16F10 and sarcoma 180 with complex 1 and performed qPCR to quantify mRNA relative levels of two genes involved in the apoptosis pathway, B-cell lymphoma 2 (BCL-2) and caspase 3 (CSP3), and two genes of the autophagic pathway (ATG5 and ATG7), once autophagy can also trigger apoptosis.