To assess whether this loss of rhVim-mediated inhibition of HPV16-PsVs infection was due to the disintegration of the rhVim/HPV complex, stability assays were performed where the pre-incubated virus/vimentin complex was added to a conditioned medium at 37 °C for up to 8 h before the addition to NIKS cells and assessment of internalisation at the 30 min time point. Here, VIM is linked to infection.