The difference between serum 25(OH)D and free 25(OH)D on glucose and GDM risk indicates that the proportion of free 25(OH)D is mainly related to muscle insulin resistance or insulin secretion, and serum 25(OH)D in circulation is not mainly mediated by free 25(OH)D, which may be related to fasting gluconeogenesis levels in the liver, and plays its role in lowering glucose levels through megalin/cubilin-mediated endocytosis through the kidney or parathyroid cells [44]. The gene discussed is INS; the disease is gestational diabetes.