Similar to studies targeting eNAMPT in preclinical models of radiation pneumonitis [21] and pulmonary hypertension [22], we evaluated the therapeutic effects of an eNAMPT-neutralizing humanized monoclonal antibody, ALT-100, in two human PCa orthotopic xenograft SCID mice models with implantation of aggressive PCa cells, DU145 or PC3, representing both PTEN-positive (DU145) and PTEN-negative (PC3) tumor subtypes. This evidence concerns the gene PTEN and posterior cortical atrophy.