CXCL10 and neoplasm: To explore the mechanism of MMP inhibition and immune enhancement therapy of TMNPs in vivo, Western blot was used to investigate the expression levels of MMP-9 (92 kDa) and CXCL10 (10 kDa) in tumor tissues collected after treatment by saline, PTX@TF, MMST/LTSLs + HT and TMNPs + HT seven times every three days, respectively, in the 4T1 tumor-bearing BALB/C mouse model (Figure 5A).