For the activation of immune cells with MMP inhibition by MMST, CXCL-10 stimulated immune cells through polarization and activation of Type 1 T-helper cells, resulting in the enhanced paracrine secretion of IFN-γ and IL-2 to facilitate the anti-tumor efficacy of TMNPs + HT (Figure 5A, Figure 6 and Figure 7). This evidence concerns the gene IL2 and neoplasm.