Subsequently, univariate and multivariate Cox regression analyses were conducted to identify independent predictors for overall survival in TCGA HCC database, and revealed that TMN stage (P < 0.001), tumor depth (P < 0.001), distant metastasis (P = 0.023), and CENPU expression (P = 0.005) were prognostic factors for overall survival in TCGA HCC cohort. The gene discussed is CENPU; the disease is hepatocellular carcinoma.