A study with colitis-induced rats found that C18:3n-3 decreased neutrophil function and infiltration and had a beneficial effect on colonic Inducible Nitric Oxide Synthase (iNOS) expression, glutathione (GSH) concentration and inflammatory stress (measured as reduced secretion of tumor necrosis factor (TNF) and mRNA level, cyclooxygenase (COX)-2 expression, lowered leukotriene B4 (LTB4) and Interleukin 6 (IL-6) production). Here, TNF is linked to colitis.