Temporal changes in the frequency of T CD4 lymphocyte populations during NAFLD progression have also been observed: in a study on MCD-fed mice, it was observed an increase in Th17 cells in the first phases of the disease, and in the NASH-fibrosis transition, while Th22 increased between the two Th17 expansions. Here, CD4 is linked to metabolic dysfunction-associated steatohepatitis.