To test this hypothesis, RNA sequencing analysis of DUXAP8-knockdown HCC cells was conducted to screen out significantly upregulated microRNAs, and the bioinformatics database, Encyclopedia of RNA Interactomes (previously known as starBase v2.0) was used, to predict the microRNAs that can target and bind to DUXAP8. In this study, two microRNAs were intersected, and three microRNAs (miR-584-5p, miR-409-3p, miR-374b-5p) were screened. Here, DUXAP8 is linked to hepatocellular carcinoma.