Epithelial–mesenchymal transition (EMT) is closely related to the migration and invasion of tumor cells, and it was found through Western blot that when DUXAP8 was knocked down, the epithelial marker E-cadherin increased and the mesenchymal marker N-cadherin and vimentin decreased in HCC cell lines (Figures 2B,D); when DUXAP8 was overexpressed, it produced the opposite effect (Figure 2F; Supplementary Figure S2D), indicating that DUXAP8 positively regulated EMT process in HCC cells. This evidence concerns the gene VIM and neoplasm.