To investigate the effect of DUXAP8 on the biological behavior of HCC cells, shRNAs, and lentiviral vectors of DUXAP8 mimics were transfected into DUXAP8 high-expressing HCC cell lines (Huh7, SNU-449) and DUXAP8 low-expressing HCC cell lines (SK-Hep-1, HCC-LM3), respectively, and the transfection efficiency was verified by RT-qPCR (Supplementary Figures S2A,B). The gene discussed is DUXAP8; the disease is hepatocellular carcinoma.