The ccRCC samples in m5C cluster A showed prominent upregulation in E2F1, miR-147B, miR-3910, miR-4261, miR-3689-3p, miR-4719, PBXIP1, and ZNF184 targeted regulation and downregulation in peptidyl modification processes, such as histone binding, peptide amino acid modification, protein autoubiquitination, ubiquitin-like protein ligase, and transferase activities (Supplementary Figure S2A). The gene discussed is E2F1; the disease is nonpapillary renal cell carcinoma.