Through this approach it was found that phosphorylation of 84 sites in 76 proteins was higher in NG2+ versus NG2− cells interacting with Col VI, while 75 sites in 58 components were found to display reduced phosphorylated, indicating that NG2-collagen VI interaction activates multiple signal transduction cascades including cell survival– and migration–promoting pathways in this fibrosarcoma model. The gene discussed is CSPG4; the disease is fibrosarcoma.