To evaluate the potential effects of CAF induction and T cell activation by collagen organization, we visualized the α-SMA and fibronectin expression of MRC-5 and NIH/3T-3 cells as well as the secretion of IL-2 of primary T cells and Jurkat T cells in 3D random and aligned collagen matrix supplement with supernatant samples of breast cancer cell MB-231. The gene discussed is ACTA1; the disease is breast cancer.