To investigate the pro-growth and pro-metastasis roles of TRIM54 in HCC progression, we used the Hep3B cell line with a medium level of TRIM54 expression for both knockdown and overexpression modifications, the knockdown experiment in TRIM54 highly expressing cells, which gradually increases metastatic potential (MHCC97H), and the overexpression experiment in the cells with low TRIM54 expression (SUN182) (Supplemental Figure 2). The gene discussed is TRIM54; the disease is hepatocellular carcinoma.