Interestingly, real-time PCR and Western blotting analyses revealed that TRIM54 was significantly overexpressed in HCC cell lines and HCC tissues at both protein and mRNA levels, compared with the two normal liver cells and two non-tumor specimens (Figures 1C, D and Supplemental Figures 1B, C), suggesting that TRIM54 is upregulated in hepatocellular carcinoma tissues. This evidence concerns the gene TRIM54 and hepatocellular carcinoma.