Laser speckle blood flow analysis and Nissl staining are one of the important methods for evaluating cerebral ischemia-reperfusion injury; after knocking down ADAM8 in cerebral ischemia-reperfusion mouse cerebral cortex, it can significantly improve the cerebral blood flow and the loss of Nissl body in cerebral ischemia-reperfusion mice (Figures 3(c) and 3(d)). Here, ADAM8 is linked to brain ischemia.