Then, cases with pattern C were classified as two subgroups according to the staining intensity: Pattern C1 (Figure 2(c)), 21-60% of tumor cells stained positive for p53 with variable intensity, and focal tumor cell nests with strong positive staining were present; pattern C2 (Figure 2(d)): 61-100% of tumor cells had diffuse and strongly positive p53 staining. This evidence concerns the gene TP53 and neoplasm.