Using the above cut-offs, three distinct immunohistochemical patterns of stained nuclei were identified based on both the percentage of positive cells and the staining intensity: pattern A (Figure 2(a)), tumor cells showed a complete absence of nuclear p53 immunolabeling with scattered weakly positive stromal cells serving as an internal reference for IHC assessment; pattern B (Figure 2(b)), 1-20% of tumor cells showed weakly positive staining in a scattered pattern; Pattern C, 21-100% tumor cells stained positive for p53. Here, TP53 is linked to neoplasm.