The comparison of the mRNA content of EVs produced by B cells from healthy donors versus patients with CLL, and stimulated or not through the TLR9 pathway, shows enrichment for the kinases of the BCR pathway, LYN, SYK, MAPK1, MAPK2, and the anti-apoptotic proteins BCL2 and BCL3 in CLL-derived EVs. Here, BCL3 is linked to B-cell chronic lymphocytic leukemia.