Zager et al. found that the expression of Cav-1 was increased in ischemia ± reperfusion–induced AKI mice and verified that the destruction of the caveolae in the damage of renal tubular epithelial cells, which causes cholesterol and Cav-1 are separated from the plasma membrane, leading to free cholesterol deposits in the lumen of the renal tubules and an increased level of urine Cav-1 (Zager et al., 2002). This evidence concerns the gene CAV1 and acute kidney injury.