Although further experimental studies are needed, with the information obtained from MRI and histologic evaluation, our present study suggested that TSTT treatment could promote long-lasting rehabilitation following ischemic stroke, as manifested by attenuating brain tissues injuries, promoting axonal remodeling accompanied with CBF restoration, boosting endogenous oligodendrogenesis, which might at least partially through activating PI3K/AKT/GSK-3β/CRMP-2 signaling pathway after ischemic stroke, all of which was pivotal for the neural repair following ischemia. This evidence concerns the gene AKT1 and ischemic stroke.