Immunologic signature analysis was further carried out and showed that the upregulated mRNAs in CRC metastasis were associated with genes upregulated in B lymphocytes and downregulated genes in comparison with DC versus Th1 cells (Figure 6E), while downregulated mRNAs in CRC metastasis were associated with genes regulated in CD4 T cells (Figure 6F). This evidence concerns the gene CD4 and colorectal carcinoma.