In summary, we found that in prostatic epithelial cells, androgen upregulates the transcription of ZFHX3 via the binding of AR to specific AREs in the ZFHX3 promoter, and the regulatory relationship between androgen/AR and ZFHX3 is valid in both mouse prostates and human prostate cancer specimens. Here, ZFHX3 is linked to Familial prostate cancer.