To test whether in vitro ASC maturation was similar to the transcriptional and morphological changes observed in the ex vivo blood and BM ASC, we sorted blood ASC (CD19+IgD−CD38+CD27+) from adults after tetanus vaccination and cultured them in the in vitro BM mimetic system (Fig S6; Nguyen et al, 2018b). This evidence concerns the gene CD27 and tetanus.