ACTA1 and Hepatic fibrosis: Moreover, in liver fibrosis, excessive accumulation of ECM is mainly due to HSC activation [8], and we observed significant increases in α-SMA (a marker of HSCs activation [25]) levels, detected by IHC (Fig. 3a), western blot (Fig. 3d) and qPCR (Fig. 3f), in the model group, while this level decreased after YQRG-H treatment.