Of note, upregulation of Ptgs2 expression and production of PGE2 were both potentiated by BMDM exposure to IFNγ prior to infection with Mtb, and the inhibitory effect of iFADS2 on Mtb-driven production of PGE2 production was maintained in IFNγ-activated macrophages (Figure 3—figure supplement 1B and C). Here, PTGS2 is linked to infection.