In the validation cohort, univariate Kaplan–Meier analyses showed that tumor stage (p = 0.001), lymph node metastasis (p = 0.0 02), UICC stage (p = 0.001), and PD-1H expression (p = 0.029) were associated with the overall survival of patients with human ESCC. The gene discussed is VSIR; the disease is esophageal squamous cell carcinoma.