Although no statistically significant association was found between PD-1H mRNA expression and overall survival in ESCC from TCGA (Figure 4A), Kaplan–Meier analyses showed that ESCC patients with high PD-1H protein expression had significantly improved overall survival (HR = 0.60; 95% CI, 0.27–0.93; p = 0.029; Figure 4B) in the exploratory cohort, especially among the G2/3 subgroup (Figures 4C,D). The gene discussed is VSIR; the disease is esophageal squamous cell carcinoma.