NR1H4 and obesity due to melanocortin 4 receptor deficiency: In summary, WGQK intervention improved HFD-induced obesity, and its potential mechanism might involve the following: (1) selectively enriched a group of gut bacteria which have been demonstrated to alleviate obesity, including Akkermansia, Lactobacillus, and Bifidobacterium; (2) increased accumulation of indole and its derivatives, which are the products of tryptophan catabolism by gut microbiota; (3) through the beneficial effects of unsaturated fatty acids; (4) inhibition of the intestinal FXR-FGF15 signaling pathway to regulate bile acids production.