Alcoholic liver disease mouse and cell models were constructed to examine the expression levels of miR-182-5p and FOXO1; dual-luciferase reporter assays were then used to explore their regulatory relationship; knockdown and overexpression experimental studies were performed to investigate the mechanism underlying the effects of the miR-182-5p/FOXO1 signaling pathway in lipid accumulation in ALD. This evidence concerns the gene FOXO1 and alcoholic liver diseases.